Are phloem sieve tubes leaky conduits supported by numerous aquaporins?
Identifieur interne : 001521 ( Main/Exploration ); précédent : 001520; suivant : 001522Are phloem sieve tubes leaky conduits supported by numerous aquaporins?
Auteurs : Ryan C. Stanfield [Canada] ; Uwe G. Hacke ; Joan Laur [Canada]Source :
- American journal of botany [ 1537-2197 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- physiologie : Aquaporines, Feuilles de plante, Phloème, Populus, Protéines végétales.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Aquaporins, Plant Proteins.
- physiology : Phloem, Plant Leaves, Populus.
Abstract
PREMISE OF THE STUDY
Aquaporin membrane water channels have been previously identified in the phloem of angiosperms, but currently their cellular characterization is lacking, especially in tree species. Pinpointing the cellular location will help generate new hypotheses of how membrane water exchange facilitates sugar transport in plants.
METHODS
We studied histological sections of balsam poplar (
KEY RESULTS
Sieve tubes of the leaf lamina, petiole, and stem were labeled with antibodies directed at PIP1s and PIP2s. While PIP2s were mostly observed in the plasma membrane, PIP1s showed both an internal membrane and plasma membrane labeling pattern.
CONCLUSIONS
The specificity and consistency of PIP2 labeling in sieve element plasma membranes points to high water exchange rates between sieve tubes and adjacent cells. The PIP1s may relocate between internal membranes and the plasma membrane to facilitate dynamic changes in membrane permeability of sieve elements in response to changing internal or environmental conditions. Aquaporin-mediated changes in membrane permeability of sieve tubes would also allow for some control of radial exchange of water between xylem and phloem.
DOI: 10.3732/ajb.1600422
PubMed: 28526726
Affiliations:
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Hacke</name><affiliation><nlm:affiliation>Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2E3, Canada; ORCID id: 0000-0002-7507-7550.</nlm:affiliation><wicri:noCountry code="subField">Canada; ORCID id: 0000-0002-7507-7550</wicri:noCountry></affiliation></author><author><name sortKey="Laur, Joan" sort="Laur, Joan" uniqKey="Laur J" first="Joan" last="Laur">Joan Laur</name><affiliation wicri:level="4"><nlm:affiliation>Centre de Recherche en Horticulture, Université Laval, Envirotron, Québec, QC G1V0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de Recherche en Horticulture, Université Laval, Envirotron, Québec, QC G1V0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28526726</idno><idno type="pmid">28526726</idno><idno type="doi">10.3732/ajb.1600422</idno><idno type="wicri:Area/Main/Corpus">001313</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001313</idno><idno type="wicri:Area/Main/Curation">001313</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001313</idno><idno type="wicri:Area/Main/Exploration">001313</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Are phloem sieve tubes leaky conduits supported by numerous aquaporins?</title><author><name sortKey="Stanfield, Ryan C" sort="Stanfield, Ryan C" uniqKey="Stanfield R" first="Ryan C" last="Stanfield">Ryan C. Stanfield</name><affiliation wicri:level="1"><nlm:affiliation>Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2E3, Canada; ORCID id: 0000-0002-7507-7550 stanfiel@ualberta.ca.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2E3</wicri:regionArea><wicri:noRegion>AB T6G 2E3</wicri:noRegion></affiliation></author><author><name sortKey="Hacke, Uwe G" sort="Hacke, Uwe G" uniqKey="Hacke U" first="Uwe G" last="Hacke">Uwe G. Hacke</name><affiliation><nlm:affiliation>Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2E3, Canada; ORCID id: 0000-0002-7507-7550.</nlm:affiliation><wicri:noCountry code="subField">Canada; ORCID id: 0000-0002-7507-7550</wicri:noCountry></affiliation></author><author><name sortKey="Laur, Joan" sort="Laur, Joan" uniqKey="Laur J" first="Joan" last="Laur">Joan Laur</name><affiliation wicri:level="4"><nlm:affiliation>Centre de Recherche en Horticulture, Université Laval, Envirotron, Québec, QC G1V0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre de Recherche en Horticulture, Université Laval, Envirotron, Québec, QC G1V0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author></analytic><series><title level="j">American journal of botany</title><idno type="eISSN">1537-2197</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aquaporins (physiology)</term><term>Phloem (physiology)</term><term>Plant Leaves (physiology)</term><term>Plant Proteins (physiology)</term><term>Populus (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aquaporines (physiologie)</term><term>Feuilles de plante (physiologie)</term><term>Phloème (physiologie)</term><term>Populus (physiologie)</term><term>Protéines végétales (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Aquaporins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Aquaporines</term><term>Feuilles de plante</term><term>Phloème</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phloem</term><term>Plant Leaves</term><term>Populus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>PREMISE OF THE STUDY</b></p><p>Aquaporin membrane water channels have been previously identified in the phloem of angiosperms, but currently their cellular characterization is lacking, especially in tree species. Pinpointing the cellular location will help generate new hypotheses of how membrane water exchange facilitates sugar transport in plants.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>We studied histological sections of balsam poplar (</p></div><div type="abstract" xml:lang="en"><p><b>KEY RESULTS</b></p><p>Sieve tubes of the leaf lamina, petiole, and stem were labeled with antibodies directed at PIP1s and PIP2s. While PIP2s were mostly observed in the plasma membrane, PIP1s showed both an internal membrane and plasma membrane labeling pattern.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The specificity and consistency of PIP2 labeling in sieve element plasma membranes points to high water exchange rates between sieve tubes and adjacent cells. The PIP1s may relocate between internal membranes and the plasma membrane to facilitate dynamic changes in membrane permeability of sieve elements in response to changing internal or environmental conditions. Aquaporin-mediated changes in membrane permeability of sieve tubes would also allow for some control of radial exchange of water between xylem and phloem.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">28526726</PMID><DateCompleted><Year>2017</Year><Month>10</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1537-2197</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><Issue>5</Issue><PubDate><Year>2017</Year><Month>May</Month></PubDate></JournalIssue><Title>American journal of botany</Title><ISOAbbreviation>Am J Bot</ISOAbbreviation></Journal><ArticleTitle>Are phloem sieve tubes leaky conduits supported by numerous aquaporins?</ArticleTitle><Pagination><MedlinePgn>719-732</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3732/ajb.1600422</ELocationID><Abstract><AbstractText Label="PREMISE OF THE STUDY" NlmCategory="OBJECTIVE">Aquaporin membrane water channels have been previously identified in the phloem of angiosperms, but currently their cellular characterization is lacking, especially in tree species. Pinpointing the cellular location will help generate new hypotheses of how membrane water exchange facilitates sugar transport in plants.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">We studied histological sections of balsam poplar (<i>Populus balsamifera</i> L.) in leaf, petiole, and stem organs. Immuno-labeling techniques were used to characterize the distribution of PIP1 and PIP2 subfamilies of aquaporins along the phloem pathway. Confocal and super resolution microscopy (3D-SIM) was used to identify the localization of aquaporins at the cellular level.</AbstractText><AbstractText Label="KEY RESULTS" NlmCategory="RESULTS">Sieve tubes of the leaf lamina, petiole, and stem were labeled with antibodies directed at PIP1s and PIP2s. While PIP2s were mostly observed in the plasma membrane, PIP1s showed both an internal membrane and plasma membrane labeling pattern.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The specificity and consistency of PIP2 labeling in sieve element plasma membranes points to high water exchange rates between sieve tubes and adjacent cells. The PIP1s may relocate between internal membranes and the plasma membrane to facilitate dynamic changes in membrane permeability of sieve elements in response to changing internal or environmental conditions. Aquaporin-mediated changes in membrane permeability of sieve tubes would also allow for some control of radial exchange of water between xylem and phloem.</AbstractText><CopyrightInformation>© 2017 Botanical Society of America.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stanfield</LastName><ForeName>Ryan C</ForeName><Initials>RC</Initials><AffiliationInfo><Affiliation>Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2E3, Canada; ORCID id: 0000-0002-7507-7550 stanfiel@ualberta.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hacke</LastName><ForeName>Uwe G</ForeName><Initials>UG</Initials><AffiliationInfo><Affiliation>Department of Renewable Resources, University of Alberta, Edmonton, AB T6G 2E3, Canada; ORCID id: 0000-0002-7507-7550.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laur</LastName><ForeName>Joan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Centre de Recherche en Horticulture, Université Laval, Envirotron, Québec, QC G1V0A6, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Am J Bot</MedlineTA><NlmUniqueID>0370467</NlmUniqueID><ISSNLinking>0002-9122</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020346">Aquaporins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020346" MajorTopicYN="N">Aquaporins</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052585" MajorTopicYN="N">Phloem</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">3D-SIM</Keyword><Keyword MajorTopicYN="N">Münch hypothesis</Keyword><Keyword MajorTopicYN="N">Populus balsamifera</Keyword><Keyword MajorTopicYN="N">aquaporin trafficking</Keyword><Keyword MajorTopicYN="N">immuno-labeling</Keyword><Keyword MajorTopicYN="N">membrane permeability</Keyword><Keyword MajorTopicYN="N">phloem transport</Keyword><Keyword MajorTopicYN="N">phloem-xylem interactions</Keyword><Keyword MajorTopicYN="N">radial water exchange</Keyword><Keyword MajorTopicYN="N">sieve tubes</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>12</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>04</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28526726</ArticleId><ArticleId IdType="pii">ajb.1600422</ArticleId><ArticleId IdType="doi">10.3732/ajb.1600422</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Québec</li></region><settlement><li>Québec (ville)</li></settlement><orgName><li>Université Laval</li></orgName></list><tree><noCountry><name sortKey="Hacke, Uwe G" sort="Hacke, Uwe G" uniqKey="Hacke U" first="Uwe G" last="Hacke">Uwe G. Hacke</name></noCountry><country name="Canada"><noRegion><name sortKey="Stanfield, Ryan C" sort="Stanfield, Ryan C" uniqKey="Stanfield R" first="Ryan C" last="Stanfield">Ryan C. Stanfield</name></noRegion><name sortKey="Laur, Joan" sort="Laur, Joan" uniqKey="Laur J" first="Joan" last="Laur">Joan Laur</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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